Immunodetection of Pneumocystis carinii in bronchoalveolar lavage specimens compared with methenamine silver stain
نویسندگان
چکیده
منابع مشابه
Utility of reflexive gomori methenamine silver and Acid-fast bacillus staining on bronchoalveolar lavage specimens.
OBJECTIVE Reflexive testing of bronchoalveolar lavage (BAL) specimens with Gomori methenamine silver (GMS) and acid-fast bacillus (AFB) stains is not routinely performed by most institutions. Instead, these stains are usually ordered to evaluate for the presence of fungal elements and/or acid-fast organisms if initial histopathologic assessment suggests the presence of these pathogens. Our inst...
متن کاملSputum induction compared with bronchoalveolar lavage by Ballard catheter to diagnose Pneumocystis carinii pneumonia.
Specimens from sputum induction (SI) are often of insufficient volume or have poor diagnostic yield for the diagnosis of Pneumocystis carinii pneumonia (PCP). The Ballard catheter for bronchoalveolar lavage (BAL) is a disposable BAL device which is passed transnasally into the airway and wedged by sensation. Thirty consecutive episodes (21 inpatient and 9 outpatient) in 28 patients positive for...
متن کاملLocal antibodies against Pneumocystis carinii in bronchoalveolar lavage fluid.
Pneumocystis carinii pneumonia (PCP) is a frequent cause of pneumonia among human immunodeficiency virus (HIV)-infected patients. Little is known, however, about the role played by humoral immunity to control the infection. This study was undertaken to elucidate the role played by local antibodies. Bronchoalveolar lavage (BAL) fluids from 18 acquired immune deficiency syndrome (AIDS) patients w...
متن کاملMonoclonal immunofluorescence compared with silver stain for investigating Pneumocystis carinii pneumonia.
Two hundred and eighty two specimens from 220 patients positive for HIV with respiratory tract symptoms, or febrile illness, or both, were examined for the presence of Pneumocystis carinii. Specimens were either induced sputum samples or bronchoalveolar lavage fluids. To establish the optimal method for laboratory diagnosis a comparison was made of detection of the organism by use of monoclonal...
متن کاملمقایسه لام های تماسی و برشهای بافتی با استفاده از روشهای رنگ آمیزی مختلف در تشخیص عفونت پنوموسیستیس کارینی
Background & Aims: Pneumocystis carinii is an opportunist extracellular microorganism. The body can defend itself against it in natural conditions. But it can cause an infection in immunodificient conditions such as HIV, Hodgkins and other immunosuppressed diseases. The aim of this study was to apply a comparison of different staining methods such as Methenamine silver, Giemsa, Gram - Weigert, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1996
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.34.3.728-730.1996